Regulatory

Part:BBa_K3054004:Design

Designed by: Dharmik Hitesh Patel   Group: iGEM19_Chalmers-Gothenburg   (2019-10-20)


pTEF1/pCIT2 dual promoter (yeast)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1427
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1427
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1427
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1427
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 241


Design Notes

Single promoters for pCIT2 and pTEF1 were extracted from genome using PCR amplification. They were then attached to each other by fusion PCR using linker as complementary sequence for fusion


Source

Saccharomyces cerevisiae genome

References